期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/ijms23116137
关键词
antimicrobial photodynamic therapy; photodynamic inactivation; digital holotomography; quantitative phase imaging; Chlorin e6; Pheophorbide a; E; coli
资金
- National Center of Science, Poland [2021/41/B/ST7/04002]
Antimicrobial photodynamic therapy (aPDT) is a promising alternative for preventing infections as bacteria cannot develop resistance to aPDT. This study demonstrated an approach to enhance aPDT effectiveness by increasing the accumulation of photosensitizers within bacterial cells using a combination of Chlorin e6 and Pheophorbide. Digital holotomography (DHT) was used to investigate the accumulation of photosensitizers and changes in the density of single-cell bacteria before and after aPDT treatment.
The prevention of biofilm formation is crucial for the limitation of bacterial infections typically associated with postoperative infections, complications in bedridden patients, and a short-term prognosis in affected cancer patients or mechanically ventilated patients. Antimicrobial photodynamic therapy (aPDT) emerges as a promising alternative for the prevention of infections due to the inability of bacteria to become resistant to aPDT inactivation processes. The aim of this study was to demonstrate the use of a functionalized combination of Chlorin e6 and Pheophorbide as a new approach to more effective aPDT by increasing the accumulation of photosensitizers (PSs) within Escherichia coli cells. The accumulation of PSs and changes in the dry mass density of single-cell bacteria before and after aPDT treatment were investigated by digital holotomography (DHT) using the refractive index as an imaging contrast for 3D label-free live bacteria cell imaging. The results confirmed that DHT can be used in complex examination of the cell-photosensitizer interaction and characterization of the efficiency of aPDT. Furthermore, the use of Pheophorbide a as an efflux pomp inhibitor in combination with Chlorin e6 increases photosensitizers accumulation within E. coli and overcomes the limited penetration of Gram-negative cells by anionic and neutral photosensitizers.
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