期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/ijms23095199
关键词
hypertension; urinary albumin excretion; long non-coding RNA; exosomes
资金
- Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III [PI12/02615, PI18/01405, PI19/01796, PI21/00249, CD18/00166, FI20/00096, PID2020-117114GBI00]
- Spanish university system [Ndegrees0-117114GBI00, ZA21-063]
- Ministry of Universities of the Government of Spain
- European Union, NextGeneration EU [ProgramNCS2021_013]
- European Regional Development Fund (ERDF)
This study identified different expression profiles of long non-coding RNAs (lncRNAs) associated with hypertension in plasma and plasma exosomes using next-generation sequencing. Analysis of gene ontology and KEGG pathways showed enrichment in biological functions related to albuminuria, providing insights into the mechanisms of albuminuria and cardiovascular damage.
Non-coding RNA (ncRNA)-mediated targeting of various genes regulates the molecular mechanisms of the pathogenesis of hypertension (HTN). However, very few circulating long ncRNAs (lncRNAs) have been reported to be altered in essential HTN. The aim of our study was to identify a lncRNA profile in plasma and plasma exosomes associated with urinary albumin excretion in HTN by next-generation sequencing and to assess biological functions enriched in response to albuminuria using GO and KEGG analysis. Plasma exosomes showed higher diversity and fold change of lncRNAs than plasma, and low transcript overlapping was found between the two biofluids. Enrichment analysis identified different biological pathways regulated in plasma or exosome fraction, which were implicated in fatty acid metabolism, extracellular matrix, and mechanisms of sorting ncRNAs into exosomes, while plasma pathways were implicated in genome reorganization, interference with RNA polymerase, and as scaffolds for assembling transcriptional regulators. Our study found a biofluid specific lncRNA profile associated with albuminuria, with higher diversity in exosomal fraction, which identifies several potential targets that may be utilized to study mechanisms of albuminuria and cardiovascular damage.
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