期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 4, 页码 881-889出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05180
关键词
Dendrobium officinale; polysaccharides; chemical structure; RAW264.7 cells; lymphocytes
资金
- HKSAR Innovation and Technology Fund (ITF), Tier 3 [ITS/311/09]
- Hong Kong Baptist University (RC-start up grant) [MPCF-001-2014/2015, FRG2/14-15/028]
A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker, of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and H-1 and C-13 NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4 -linked beta-D-mannopyranosyl and beta-D-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-alpha secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale.
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