期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/ijms23042100
关键词
COVID-19; SARS-CoV-2 RNA; mitochondria; placenta; padlock
In this pilot study, the researchers optimized the padlock assay to visualize the genomic and subgenomic regions of SARS-CoV-2 in placental samples from a confirmed COVID-19 case. They found a significant overlap between SARS-CoV-2 RNA and mitochondria in trophoblastic cells, suggesting a potential reprogramming of mitochondrial activity in the maternal-fetal interface during SARS-CoV-2 infection.
The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal-fetal interface.
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