期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/ijms23115959
关键词
Arabidopsis; callose; meiosis; pollen development; exine; Rhomboid
资金
- Nagoya University Research Fund
- Japanese Ministry of Education, Culture, Sports, Science and Technology (the Grant for Biodiversity Research of the 21st Century COE (A14)
- Japan Society for the Promotion of Science for Young Scientists
- Swiss National Science Foundation [31003A_182318]
- JST CREST [JPMJCR16O3]
- MEXT KAKENHI Japan [16H06469]
- Burroughs Wellcome Fund
- NIH [AI066025]
- National Science Foundation [IOB-0543923, MCB-0548525]
- [14036220]
- Grants-in-Aid for Scientific Research [16H06469] Funding Source: KAKEN
Fertilization is a key event in sexually reproducing plants. A study on Arabidopsis thaliana identified a mutant, kompeito-1 (kom-1), which showed defects in pollen wall patterning and pollen-stigma adhesion. The study found that the KOM gene is involved in the regulation of CalS5 protein accumulation, which in turn affects callose accumulation and pollen wall formation.
Fertilization is a key event for sexually reproducing plants. Pollen-stigma adhesion, which is the first step in male-female interaction during fertilization, requires proper pollen wall patterning. Callose, which is a beta-1.3-glucan, is an essential polysaccharide that is required for pollen development and pollen wall formation. Mutations in CALLOSE SYNTHASE 5 (CalS5) disrupt male meiotic callose accumulation; however, how CalS5 activity and callose synthesis are regulated is not fully understood. In this paper, we report the isolation of a kompeito-1 (kom-1) mutant defective in pollen wall patterning and pollen-stigma adhesion in Arabidopsis thaliana. Callose was not accumulated in kom-1 meiocytes or microspores, which was very similar to the cals5 mutant. The KOM gene encoded a member of a subclass of Rhomboid serine protease proteins that lacked active site residues. KOM was localized to the Golgi apparatus, and both KOM and CalS5 genes were highly expressed in meiocytes. A 220 kDa CalS5 protein was detected in wild-type (Col-0) floral buds but was dramatically reduced in kom-1. These results suggested that KOM was required for CalS5 protein accumulation, leading to the regulation of meiocyte-specific callose accumulation and pollen wall formation.
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