期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 11, 页码 2269-2279出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b00030
关键词
intracellular Ca2+ concentration; cytokine secretion; ginger (Zingiber officiale Roscoe); gingerols; IFN-gamma; T lymphocytes; TRPV1
In a pilot study with two volunteers, the main pungent and bioactive ginger (Zingiber officinale Roscoe) compounds, the gingerols, were quantitated in human plasma after ginger tea consumption using a newly established HPLC-MS/MS(ESI) method on the basis of stable isotope dilution assays. Limits of quantitation for [6]-, [8]-, and [10]-gingerols were determined as 7.6, 3.1, and 4.0 nmol/L, respectively. The highest plasma concentrations of [6]-, [8]-, and [10]-gingerols (42.0, 5.3, and 4.8 nmol/L, respectively) were reached 30-60 min after ginger tea intake. Incubation of activated human T lymphocytes with gingerols increased the intracellular Ca2+ concentration as well as the IFN-gamma secretion by about 20-30%. This gingerolinduced increase of IFN-gamma secretion could be blocked by the specific TRPV1 antagonist SB-366791. The results of the present study point to an interaction of gingerols with TRPV1 in activated T lymphocytes leading to an augmentation of IFN-y secretion.
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