Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells

High-energy industrial processes have been associated with particle release into workplace air that can adversely affect workers' health. The present study assessed the toxicity of incidental fine (PGFP) and nanoparticles (PGNP) emitted from atmospheric plasma (APS) and high-velocity oxy-fuel (HVOF) thermal spraying. Lactate dehydrogenase (LDH) release, 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) metabolisation, intracellular reactive oxygen species (ROS) levels, cell cycle changes, histone H2AX phosphorylation (gamma-H2AX) and DNA damage were evaluated in human alveolar epithelial cells at 24 h after exposure. Overall, HVOF particles were the most cytotoxic to human alveolar cells, with cell viability half-maximal inhibitory concentration (IC50) values of 20.18 mu g/cm(2) and 1.79 mu g/cm(2) for PGFP and PGNP, respectively. Only the highest tested concentration of APS-PGFP caused a slight decrease in cell viability. Particle uptake, cell cycle arrest at S + G(2)/M and gamma-H2AX augmentation were observed after exposure to all tested particles. However, higher levels of gamma-H2AX were found in cells exposed to APS-derived particles (similar to 16%), while cells exposed to HVOF particles exhibited increased levels of oxidative damage (similar to 17% tail intensity) and ROS (similar to 184%). Accordingly, APS and HVOF particles seem to exert their genotoxic effects by different mechanisms, highlighting that the health risks of these process-generated particles at industrial settings should not be underestimated.

Automated summary

This study assessed the toxicity of particles emitted from atmospheric plasma and high-velocity oxy-fuel thermal spraying, and found that they exerted different cytotoxic and genotoxic effects on human alveolar cells. The results emphasize the importance of considering the health risks of these process-generated particles in industrial settings.