期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 49, 期 5, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2022.5127
关键词
gentamicin; ototoxicity; 2,3,4 ',5-tetrahydroxystilbene-2-O-beta-D-glucoside; UB/OC-2 cochlear cells; autophagy
资金
- Buddhist Tzu Chi Medical Foundation [TCMF-MP 108-01-01, TCMF-CP 111-09, TCMF-A 108-03]
- Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation [TTCRD 110-03, TTCRD 110-24]
This study investigated the otoprotective mechanism of 2,3,4',5-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) in attenuating gentamicin ototoxicity. THSG was found to decrease gentamicin-induced ototoxicity in UB/OC-2 cochlear cells via regulating the autophagic signaling involving Sesn2/AMPK/mTOR pathway.
Gentamicin is an important aminoglycoside antibiotic used in the treatment of gram-negative bacterial infections, but nephrotoxicity and ototoxicity reduce its utility. The autophagy pathway is involved in damage of auditory hair cells. With the aim of developing new strategies for attenuating gentamicin ototoxicity, the present study investigated the otoprotective mechanism of 2,3,4',5-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) in vitro using the mouse cochlear cell line UB/OC-2. MTT assay demonstrated that gentamicin reduced UB/OC-2 cell viability and western blotting showed that gentamicin upregulated autophagy-related proteins, such as Beclin, autophagy related 5 and LC3-II. THSG significantly attenuated gentamicin-induced cytotoxicity, clearly reduced LDH release observed by LDH assay and decreased the expression of autophagy-related proteins. Reverse-transcription-quantitative (RT-q) PCR and western blotting showed that THSG against gentamicin-induced autophagy via suppressing the expression of Sesn2, at both the mRNA and protein level and a possible involvement of AMP-activated protein kinase (AMPK)/mTOR signaling response. Collectively, the present study demonstrated that THSG decreased gentamicin-induced ototoxicity in UB/OC-2 cochlear cells via the autophagic signaling in regulating Sesn2/AMPK/mTOR pathway. These results suggested that THSG might be a new therapeutic agent with the potential to attenuate gentamicin ototoxicity.
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