期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 45, 页码 8678-8683出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b03732
关键词
furazolidone; 3-amino-2-oxazolidinone; immunochromatographic test strip; quantum dot; biosensor; animal tissue
资金
- Scientific and Technological Research Project of Chongqing China [cstc2016shmszx80069]
- China Scholarship Council (CSC) [201608505051]
- National Natural Science Foundation of China [31671939]
To monitor the levels of furazolidone in edible animal tissues, a fluorescent sensor was developed for the determination of 3-amino-2-oxazolidinone (AOZ), the metabolite of furazolidone, featuring an immunochromatographic test strip assay (ITSA) integrated with a quantum dot (QD) label. The optimal QD-based ITSA sensor exhibits good dynamic linear detection for AOZ over the range of 0.1-100 mu g/L, with a 50% inhibitory concentration (IC50) of 1.06 mu g/L. The decision limit and the detection capability were 0.14-0.15 and 0.27-0.33 mu g/kg, respectively, for this analyte using the QD-based ITSA sensor. These values represent an improvement over a previously reported gold nanoparticle-based immunochromatographic assay. The recoveries of AOZ in kinds of animal tissues were between 76.3 and 98.4% at the levels of 1.0, 5.0, and 10.0 mu g/kg. The performance and practicality of our QD-based fluorescent immunosensor were confirmed by commercial ELISA kit and LC-MS/MS. In conclusion, the proposed sensor was a feasible detection method for AOZ analysis on site.
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