4.7 Article

Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats

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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 25, 页码 5180-5187

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b01669

关键词

Wistar; SHR; infarct size; mitochondria; polyphenols

资金

  1. National University of La Plata of Argentina [M-169]

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Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global ischemia, and 60 min reperfusion (R). Other hearts were treated with PCE at the onset of R Infarct size, the reduced glutathione (GSH), and the expression of phospho-Akt, P-GSK-3 beta, and P-eNOS were assessed. In isolated mitochondria, the Ca2+-mediated response of mitochondrial permeability transition pore (mPTP), membrane potential (Delta Psi m), and superoxide production were determined. PCE decreased infarct size, partly preserved GSH, increased the P-Akt, P-GSK-3 beta, and P-eNOS contents, improved mPTP response to Ca2+, decreased the superoxide production, and restored Delta Psi m. These data show that PCE decreases the cardiac postischemic damage in W rats and SHR and suggest that Akt/GSK-3 beta/eNOS dependent pathways are involved.

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