期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 17, 页码 3353-3361出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b00559
关键词
Brassica oleracea var. italica; broccoli leaf; learning and memory function; amyloid beta; oxo-dihydroxy-octadecenoic acid; trihydroxy-octadecenoic acid
资金
- Ministry of Agriculture Food and Rural Affairs [113023-3]
- National Research Foundation (NRF) of Korea - Ministry of Education [NRF-2015R1D1A3A01015931]
- Small and Medium Business Administration, Republic of Korea [2014-S2181697]
- BK21 Plus program (MEST)
To examine the antiamnesic effects of broccoli (Brassica oleracea var. italica) leaves, we performed. in vitro and in vivo tests on amyloid beta (A beta)-induced neurotoxicity. The chloroform fraction from broccoli leaves (CBL) showed a remarkable neuronal cell-protective effect and an inhibition against acetylcholinesterase (AChE). The ameliorating-effect of CBL on A beta(1-42)-induced learning and memory impairment was evaluated by Y-maze, passive avoidance, and Morris water maze tests. The results indicated improving cognitive function in the CBL group. After the behavioral tests, antioxidant effects were detected by superoxide dismutase (SOD), oxidized glutathione (GSH)/total GSH, and malondialdehyde (MDA) assays, and inhibition against AChE was also presented in the brain. Finally, oxo-dihydroxy-octadecenoic acid (oxo-DHODE) and trihydroxy-octadecenoic acid (THODE) as main compounds were identified by quadrupole time-of-flight ultraperformance liquid chromatography (Q-TOF UPLC-MS) analysis. Therefore, our studies suggest that CBL could be used as a natural resource for ameliorating A beta(1-42)-induced learning and memory impairment.
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