期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 18, 页码 3669-3677出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05322
关键词
food authentication; species identification; mass spectrometry; proteomics; spectral libraries
资金
- Dutch Ministry of Economic Affairs
- Dutch Organization of Scientific Research (NWO) via VIDI grant [917.11.398]
Proteomics methodology has seen increased application in food authentication, including tandem mass spectrometry of targeted species-specific peptides in raw, processed, or mixed food products. We have previously described an alternative principle that uses untargeted data acquisition and spectral library matching, essentially spectral counting, to compare and identify samples without the need for genomic sequence information in food species populations. Here, we present an interlaboratory comparison demonstrating how a method based on this principle performs in a realistic context. We also increasingly challenge the method by using data from different types of mass spectrometers, by trying to distinguish closely related and commercially important flatfish, and by analyzing heavily contaminated samples. The method was found to be robust in different laboratories, and 94-97% of the analyzed samples were correctly identified, including all processed and contaminated samples.
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