期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 19, 页码 3908-3915出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b01497
关键词
SQX; UCNPs; MMPMs; fluorescence immunoassay; animal-derived foods
资金
- International Science and Technology Cooperation Program of China [2014DFR30350]
- National Natural Science Foundation of China [31201353]
- Tianjin Municipal Science and Technology Commission [13JCQNJC15300]
- Ministry of Science and Technology of the People's Republic of China [2012BAD28B05]
A novel fluorescence immunoassay for detecting sulfaquinoxaline (SQX) in animal-derived foods was developed using NaYF4:Yb/Tm upconversion nanoparticles (UCNPs) conjugated with antibodies as fluorescence signal probes, and monodisperse magnetic polystyrene microspheres (M.MPMs) modified with coating antigen as immune-sensing capture probes for trapping and separating the signal probes. Based on a competitive immunoassay format, the detection limit of the proposed method for detecting SQX was 0.1 mu g L-1 in buffer and 0.5 mu g kg(-1) in food samples. The recoveries of SQX in spiked samples ranged from 69.80 to 133.00%, with coefficients of variation of 0.24-25.06%. The extraction procedure was fast, simple, and environmentally friendly, requiring no organic solvents. In particular, milk samples can be analyzed directly after simple dilution. This method has appealing properties, such as sensitive fluorescence response, a simple and fast extraction procedure, and environmental friendliness, and could be applied to detecting SQX in animal-derived foods.
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