期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 13, 页码 2709-2718出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05904
关键词
alpha-amylase; resistant starch; Bifidobacterium longum; slowly digestible starch
资金
- Hallym University Specialization Fund [HRF-S-13]
- Basic Science Research Program of the National Research Foundation [NRF-2014-0009695]
- Korean government
In this study, a novel alpha-amylase was cloned from Bifidobacterium longum and named BiLA. The enzyme exhibited optimal activity at 20 degrees C and a pH value of 5.0. Kinetic analysis using various carbohydrate substrates revealed that BiLA had the highest k(cat)/K-m value for amylose. Interestingly, analysis of the enzymatic reaction products demonstrated that BiLA specifically catalyzed the hydrolysis of oligosaccharides and starches up to G5 from the nonreducing ends. To determine whether BiLA can be used to generate slowly digestible starch (SDS), starch was treated with BiLA, and the kinetic parameters were analyzed using porcine pancreatic alpha-amylase (PPA) and amyloglucosidase (AMG). Compared to normal starch, BiLA-treated starch showed lower k(cat)/K-m values with PPA and AMG, suggesting that BiLA is a potential candidate for the production of SDS.
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