期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 41, 页码 7874-7881出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b03332
关键词
gentamicin; MWCNTs; filtration
资金
- National Natural Science Foundation of China [31502118, 31170386, 31570414]
- China Postdoctoral Science Foundation [2013M541606]
- Natural Science Fund Project of Jiangsu Province [BK20130507]
- Scientific Research Funds in Jiangsu University [13JDG016]
- Environmental Chemistry and Ecotoxicology State Key Laboratory Fund [KF2014-02]
- Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment
Current methods to detect gentamicin (GEN), a broad-spectrum antibiotic that causes ototoxicity and nephrotoxicity when present in excess, have several limitations. Hence, we have developed two methods using multi-walled carbon nanotubes (MWCNTs) as a solid support to detect GEN. Hybridoma cells (2D12) producing high-sensitivity antibodies against GEN were established. The goat anti-mouse antibody was immobilized on MWCNTs directly or using bifunctional polyethylene glycol as a linker. On the basis of the physical characteristics of MWCNTs, a quantitative method involving centrifugation separation and a qualitative method involving filtration separation were established. Various experimental parameters were optimized for GEN detection, and recovery tests were performed. For the quantitative method, the limit of detection (LOD) was 0.048 ng/mL, whereas for the qualitative method, a LOD of 0.1 ng/mL was observed by the naked eye. The proposed immunoassays were applied to commercial milk samples. Thus, these methods show potential application for the detection of GEN.
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