4.7 Article

Use of Carbon Nanotubes as a Solid Support To Establish Quantitative (Centrifugation) and Qualitative (Filtration) Immunoassays To Detect Gentamicin Contamination in Commercial Milk

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 41, 页码 7874-7881

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b03332

关键词

gentamicin; MWCNTs; filtration

资金

  1. National Natural Science Foundation of China [31502118, 31170386, 31570414]
  2. China Postdoctoral Science Foundation [2013M541606]
  3. Natural Science Fund Project of Jiangsu Province [BK20130507]
  4. Scientific Research Funds in Jiangsu University [13JDG016]
  5. Environmental Chemistry and Ecotoxicology State Key Laboratory Fund [KF2014-02]
  6. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment

向作者/读者索取更多资源

Current methods to detect gentamicin (GEN), a broad-spectrum antibiotic that causes ototoxicity and nephrotoxicity when present in excess, have several limitations. Hence, we have developed two methods using multi-walled carbon nanotubes (MWCNTs) as a solid support to detect GEN. Hybridoma cells (2D12) producing high-sensitivity antibodies against GEN were established. The goat anti-mouse antibody was immobilized on MWCNTs directly or using bifunctional polyethylene glycol as a linker. On the basis of the physical characteristics of MWCNTs, a quantitative method involving centrifugation separation and a qualitative method involving filtration separation were established. Various experimental parameters were optimized for GEN detection, and recovery tests were performed. For the quantitative method, the limit of detection (LOD) was 0.048 ng/mL, whereas for the qualitative method, a LOD of 0.1 ng/mL was observed by the naked eye. The proposed immunoassays were applied to commercial milk samples. Thus, these methods show potential application for the detection of GEN.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据