期刊
CANCER BIOLOGY & THERAPY
卷 16, 期 3, 页码 460-465出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15384047.2015.1009269
关键词
apoptosis; colorectal cancer; Fibersol-2; p53; resistant maltodextrin; reactive oxygen species; BAX; Bcl-2-associated X protein; MFI; mean fluorescence intensity; mTOR; mammalian target of rapamycin; PARP; poly ADP reibose polymerase; ROS; reactive oxygen species
类别
资金
- Matsutani America Cancer Research Fund
- National Institutes of Health [R01CA79892, R01CA90631, 5P30CA16056]
- Susan G. Komen Foundation
Resistant maltodextrin Fibersol-2 is a soluble and fermentable dietary fiber that is Generally Recognized As Safe (GRAS) in the United States. We tested whether Fibersol-2 contains anti-tumor activity. Human colorectal cancer cell line, HCT116, and its isogenic cells were treated with FIbersol-2. Tumor growth and tumorigenesis were studied in vitro and in vivo. Apoptotic pathway and generation of reactive oxygen species (ROS) were investigated. We discovered that Fibersol-2 significantly inhibits tumor growth of HCT116 cells by inducing apoptosis. Fibersol-2 strongly induces mitochondrial ROS and Bax-dependent cleavage of caspase 3 and 9, which is shown by isogenic HCT116 variants. Fibersol-2 induces phosphorylation of Akt, mTOR in parental HCT116 cells, but not in HCT116 deficient for Bax or p53. It prevents growth of tumor xenograft without any apparent signs of toxicity in vivo. These results identify Fibersol-2 as a mechanism-based dietary supplement agent that could prevent colorectal cancer development.
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