DNA methylation testing with S5 for triage of high-risk HPV positive women

Methylation of host and viral genes is promising for triage of women with high-risk human papillomavirus infections (hrHPV). Using a population-based sample of hrHPV positive women with cervical biopsies within 12 months after cervical screening, the clinical value of the S5 methylation classifier (S5), HPV genotyping and cytology were compared as potential triage tests, for outcomes of cervical intraepithelial neoplasia (CIN) grade 3 or greater (CIN3+), CIN2+ and CIN2, and the area under the curve (AUC) calculated. S5 scores increased with histopathology severity (P-trend < .001). For CIN3+, the AUC was 0.780 suggesting S5 provides good discrimination between

Automated summary

Methylation of host and viral genes shows promise in stratifying women with high-risk human papillomavirus infections. The study compared the clinical value of the S5 methylation classifier, HPV genotyping, and cytology as potential triage tests, and found that the S5 classifier performed well in discriminating CIN3+.