期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 206, 期 -, 页码 642-652出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.02.187
关键词
Isatis indigotica Fort.; Polysaccharides; Pectin; Structural analysis
资金
- National Natural Science Foundation of China [31870796]
- Scientific and Technologic Foundation of Jilin Province [20200201004JC]
- Fundamental Research Funds for the Central Universities [2412020ZD001]
- Innovation Platform Project of Qinghai province [2021-ZJ-T02]
Water-soluble polysaccharides were isolated from Isatis indigotica Fort. leaves and roots, and their structural features were studied and compared. The major pectic polysaccharides from the leaves and roots were found to be rich in RG-I and HG domains, respectively, with noticeable differences in their composition.
Water-soluble polysaccharides were isolated from the leaves and roots of Isatis indigotica Fort., and their structural features were studied and compared. One neutral polysaccharide fraction (WFIP-N) and three pectin fractions (WFIP-A-A, WFIP-A-B and WFIP-A-C) were obtained from the leaves, and one neutral polysaccharide fraction (WRIP-N) and two pectin fractions (WRIP-A-A and WRIP-A-B) were obtained from the roots. WFIP-A-B (Mw = 34.6 kDa) and WRIP-A-B (Mw = 29.9 kDa) were the major pectic polysaccharides. Monosaccharide composition, FT-IR, enzymatic hydrolysis, NMR and methylation analysis indicated that both WFIP-A-B and WRIP-A-B are composed of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II) and homogalacturonan (HG) domains with mass ratios of 1.5:1.0:0.4 and 0.3:1.0:1.7, respectively. WFIP-A-B and WRIP-A-B were found to be rich in RG-I and HG domains, respectively, and mainly contained type II arabinogalactan (AG-II) and alpha-L-1,5-arabinan side chains, but those in WRIP-A-B were more numerous and longer. Our results provide structural features and differences between these polysaccharides which will help to elucidate their functional differences.
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