期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 201, 期 -, 页码 143-157出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.12.122
关键词
Sulfated fucans; Brown algae; Fucanase
资金
- Agence Nationale de la Recherche (ANR) [ANR-18-CE43-0003-01]
- ANR Phenome-Emphasis-FR [ANR-11-INBS- 0012]
This study developed a reliable and cost-effective protocol for the preparation of sulfated fucans from brown algae. The structure of sulfated fucans was analyzed and their biological activities were explored. The findings provide a foundation for further development and utilization of sulfated fucans.
Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules. To learn more on their structure and to analyze and exploit their biological activities, there is a growing need to develop reliable and cost effective protocols for their preparation. In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans. Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC). Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight. Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity. Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans. This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.
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