期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 211, 期 -, 页码 150-158出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.05.078
关键词
Ovalbumin (OVA); Polysaccharide; Emulsion
资金
- Science and Technology Planning Project of Guangzhou, China [202102010451]
- Undergraduate Innovation Training Program of Guangzhou University [XJ202011078016]
- Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences [202108TD]
This study explored the mechanism of interactions between polysaccharides and ovalbumin-ferulic acid (OVA-FA) and the effect of polysaccharides on the stability of OVA-FA-stabilized emulsions. The results showed that the non-covalent interactions between polysaccharides and OVA-FA led to changes in the protein structure and improved the stability of the emulsions.
This study explored the mechanism underlying the interactions between polysaccharides and ovalbumin-ferulic acid (OVA-FA) and the effect of polysaccharides on OVA-FA-stabilized emulsions. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) were used to study the polysaccharide OVA-FA interactions mechanism and to resolve the changes in the protein secondary structure and crystal structure. OVA-FApolysaccharide-stabilized emulsions were studied using confocal laser scanning microscopy (CLSM), and their rheological properties and stability were determined. The results showed that the non-covalent interactions between polysaccharides and OVA-FA led to an increase in the beta-sheet content of OVA and a decrease in the alpha-helix and random coil contents. The stability of the OVA-FA-polysaccharide-stabilized emulsions was better compared with that of the OVA-FA-stabilized emulsions. By comparing the different OVA-FA-polysaccharidestabilized emulsions, we observed that OVA-FA-agar did not stabilize the emulsion well, while the OVA-FASA- and OVA-FA-KC-stabilized emulsions had good elasticity, and the microstructure and storage stability of the OVA-FA-KC-stabilized emulsion were better. Our findings provide a new perspective for the application of OVA-FA-KC in complex food emulsions.
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