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Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica
Oveimar Barbosa et al.
PROCESS BIOCHEMISTRY (2012)
The slow-down of the CALB immobilization rate permits to control the inter and intra molecular modification produced by glutaraldehyde
Oveimar Barbosa et al.
PROCESS BIOCHEMISTRY (2012)
Digital microfluidic hydrogel microreactors for proteomics
Vivienne N. Luk et al.
PROTEOMICS (2012)
Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes
Rafael C. Rodrigues et al.
ADVANCED SYNTHESIS & CATALYSIS (2011)
Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance
Cristina Garcia-Galan et al.
ADVANCED SYNTHESIS & CATALYSIS (2011)
Metagenomic Analyses: Past and Future Trends
Carola Simon et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2011)
Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance
Karel Hernandez et al.
ENZYME AND MICROBIAL TECHNOLOGY (2011)
Biotransformations in microstructured reactors: more than flowing with the stream?
Juan M. Bolivar et al.
TRENDS IN BIOTECHNOLOGY (2011)
Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis
Junfeng Ma et al.
ANALYTICAL CHEMISTRY (2010)
Enzyme technology for precision functional food ingredient processes
Anne S. Meyer
FOODS FOR HEALTH IN THE 21ST CENTURY: A ROAD MAP FOR THE FUTURE (2010)
Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives
G. Volpato et al.
CURRENT MEDICINAL CHEMISTRY (2010)
Metagenomic era for biocatalyst identification
Lucia Fernandez-Arrojo et al.
CURRENT OPINION IN BIOTECHNOLOGY (2010)
Total on-line analysis of a target protein from plasma by immunoextraction, digestion and liquid chromatography-mass spectrometry
A. Cingoez et al.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2010)
Vinyl sulfone functionalized silica: a ready to use'' pre-activated material for immobilization of biomolecules
Mariano Ortega-Munoz et al.
JOURNAL OF MATERIALS CHEMISTRY (2010)
Immobilization of Geobacillus pallidus RAPc8 nitrile hydratase (NHase) reduces substrate inhibition and enhances thermostability
Idan Chiyanzu et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2010)
Enzymatic Catalyzed Synthesis and Triggered Gelation of Ionic Peptides
Jean-Baptiste Guilbaud et al.
LANGMUIR (2010)
Vinyl sulfone: a versatile function for simple bioconjugation and immobilization
Julia Morales-Sanfrutos et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2010)
Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports
Juan M. Bolivar et al.
PROCESS BIOCHEMISTRY (2010)
Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology
Justin M. Chalker et al.
CHEMISTRY-AN ASIAN JOURNAL (2009)
Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation
Roberto Fernandez-Lafuente
ENZYME AND MICROBIAL TECHNOLOGY (2009)
HYDROPHILIC DIAZIRINE POLYMER FOR ONE-STEP PHOTO-FABRICATION OF PROTEINS ON POLYPROPYLENE SURFACE
Takenori Tomohiro et al.
HETEROCYCLES (2009)
Peptide inhibitor modified magnetic particles for pepsin separation
Michaela Filuszova et al.
JOURNAL OF SEPARATION SCIENCE (2009)
Hydrophobic mesoporous materials for immobilization of enzymes
Mina Park et al.
MICROPOROUS AND MESOPOROUS MATERIALS (2009)
Immobilization-stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination
Rafael C. Rodrigues et al.
PROCESS BIOCHEMISTRY (2009)
Pepsin immobilized in dextran-modified fused-silica capillaries for on-line protein digestion and peptide mapping
E. C. A. Stigter et al.
ANALYTICA CHIMICA ACTA (2008)
Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose
Gloria Fernandez-Lorente et al.
BIOMACROMOLECULES (2008)
Application and properties of butyl acrylate/pentaerythrite triacrylate copolymers and cellulose-based Granocel as carriers for trypsin immobilization
Jolanta Bryjak et al.
COLLOIDS AND SURFACES B-BIOINTERFACES (2008)
In-line system containing porous polymer monoliths for protein digestion with immobilized pepsin, peptide preconcentration and nano-liquid chromatography separation coupled to electrospray ionization mass spectroscopy
Laurent Geiser et al.
JOURNAL OF CHROMATOGRAPHY A (2008)
Active Biocatalysts Based on Pepsin Immobilized in Mesoporous SBA-15
Haresh G. Manyar et al.
JOURNAL OF PHYSICAL CHEMISTRY C (2008)
Continuous preparation of two opioid peptides and recycling of organic solvent using liquid/liquid extraction coupled with aluminium oxide column during haemoglobin hydrolysis by immobilized pepsin
Renato Froidevaux et al.
PROCESS BIOCHEMISTRY (2008)
Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis
Junfeng Ma et al.
JOURNAL OF SEPARATION SCIENCE (2007)
Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries
Jana Krenkova et al.
JOURNAL OF CHROMATOGRAPHY A (2007)
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo et al.
ENZYME AND MICROBIAL TECHNOLOGY (2007)
Expanding the diversity of chemical protein modification allows post-translational mimicry
Sander I. van Kasteren et al.
NATURE (2007)
Mixed ion exchange supports as useful ion exchangers for protein purification:: Purification of penicillin G acylase from Escherichia coli
Manuel Fuentes et al.
BIOMACROMOLECULES (2007)
Immobilization of pepsin on chitosan beads
Gamze Durgun Altun et al.
FOOD CHEMISTRY (2007)
Investigations of reaction kinetics for immobilized enzymess - Identification of parameters in the presence of diffusion limitation
Wouter R. Berendsen et al.
BIOTECHNOLOGY PROGRESS (2006)
Study of a continuous reactor for selective solvent extraction of haemorphins in the course of peptic haemoglobin hydrolysis
Renato Froidevaux et al.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2006)
Glyoxyl agarose:: A fully inert and hydrophilic support for immobilization and high stabilization of proteins
Cesar Mateo et al.
ENZYME AND MICROBIAL TECHNOLOGY (2006)
Enhancing protease enantioselectivity by ionic liquids based on chiral- or ω-amino acids
Hua Zhao et al.
TETRAHEDRON-ASYMMETRY (2006)
Monolithic materials - Promises, challenges, achievements
F Svec et al.
ANALYTICAL CHEMISTRY (2006)
Photonic activation of disulfide bridges achieves oriented protein immobilization on biosensor surfaces
MT Neves-Petersen et al.
PROTEIN SCIENCE (2006)
Recent trends in protease-catalyzed peptide synthesis
C Lombard et al.
PROTEIN AND PEPTIDE LETTERS (2005)
Microbial proteases in peptide synthesis: approaches and applications
D Kumar et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2005)
Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports
F López-Gallego et al.
JOURNAL OF BIOTECHNOLOGY (2005)
Increasing the binding strength of proteins to PEI coated supports by immobilizing at high ionic strength
BCC Pessela et al.
ENZYME AND MICROBIAL TECHNOLOGY (2005)
Use of a protease-modified-alumina complex to design a continuous stirred tank reactor for producing bioactive hydrolysates
EL Ticu et al.
PROCESS BIOCHEMISTRY (2005)
Immobilization of pepsin on an acrylamide/2-hydroxyethyl methacrylate copolymer and its use in casein hydrolysis
SP Shukla et al.
JOURNAL OF APPLIED POLYMER SCIENCE (2005)
Metagenomics and industrial applications
P Lorenz et al.
NATURE REVIEWS MICROBIOLOGY (2005)
Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports
F López-Gallego et al.
JOURNAL OF BIOTECHNOLOGY (2005)
Dextran aldehyde coating of glucose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles
L Betancor et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2005)
Free-standing nanogold membranes as scaffolds for enzyme immobilization
S Phadtare et al.
LANGMUIR (2004)
Ion exchange using poorly activated supports, an easy way for purification of large proteins
BCC Pessela et al.
JOURNAL OF CHROMATOGRAPHY A (2004)
A kinetic study of bovine haernoglobin hydrolysis by pepsin immobilized on a functionalized alumina to prepare hydrolysates containing bioactive peptides
EL Ticu et al.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2004)
Glide: A new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy
RA Friesner et al.
JOURNAL OF MEDICINAL CHEMISTRY (2004)
Affinity chromatography of porcine pepsin and pepsinogen using immobilized ligands derived from the specific substrate for this enzyme
J Frydlová et al.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2004)
Enzyme immobilization onto micro-sized modified PMMA microspheres
SJ Li et al.
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY (2004)
Immobilization of lactase from Kluyveromyces lactis greatly reduces the inhibition promoted by glucose.: Full hydrolysis of lactose in milk
C Mateo et al.
BIOTECHNOLOGY PROGRESS (2004)
Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextran
M Fuentes et al.
BIOTECHNOLOGY PROGRESS (2004)
Use of chemically modified PMMA microspheres for enzyme immobilization
SJ Li et al.
BIOSYSTEMS (2004)
Immobilized microfluidic enzymatic reactors
J Krenková et al.
ELECTROPHORESIS (2004)
Prevention of interfacial inactivation of enzymes by coating the enzyme surface with dextran-aldehyde
L Betancor et al.
JOURNAL OF BIOTECHNOLOGY (2004)
Continuous production of wheat gluten peptide with foaming properties using immobilized enzymes
H Motoi et al.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY (2004)
N-terminal modifications increase the neutral-pH stability of pepsin
BC Bryksa et al.
BIOCHEMISTRY (2003)
Modified proteases for peptide synthesis in organic media
IY Filippova et al.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY (2003)
The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition -: Complete hydrolysis of lactose in dairy products
BCC Pessela et al.
ENZYME AND MICROBIAL TECHNOLOGY (2003)
Biotechnological prospects from metagenomics
PD Schloss et al.
CURRENT OPINION IN BIOTECHNOLOGY (2003)
Direct assembly of gold nanoparticle shells on polyurethane microsphere cores and their application as enzyme immobilization templates
S Phadtare et al.
CHEMISTRY OF MATERIALS (2003)
Gold nanoparticles assembled on amine-functionalized Na-Y zeolite: A biocompatible surface for enzyme immobilization
K Mukhopadhyay et al.
LANGMUIR (2003)
Dispelling the myths - Biocatalysis in industrial synthesis
HE Schoemaker et al.
SCIENCE (2003)
Protease-catalyzed peptide synthesis on solid support
RV Ulijn et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2002)
Epoxy sepabeads:: A novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment
C Mateo et al.
BIOTECHNOLOGY PROGRESS (2002)
Physiological study of pH stability and sensitivity to pepsin of human gastric lipase
E Ville et al.
DIGESTION (2002)
N-terminal portion acts as an initiator of the inactivation of pepsin at neutral pH
T Tanaka et al.
PROTEIN ENGINEERING (2001)
In vitro refolding of porcine pepsin immobilized on agarose beads
E Kurimoto et al.
JOURNAL OF BIOCHEMISTRY (2001)
Multifunctional epoxy supports:: A new tool to improve the covalent immobilization of proteins.: The promotion of physical adsorptions of proteins on the supports before their covalent linkage
C Mateo et al.
BIOMACROMOLECULES (2000)
Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit™ L-100
M Sardar et al.
ENZYME AND MICROBIAL TECHNOLOGY (2000)
Mass spectrometry and immobilized enzymes for the screening of inhibitor libraries
MT Cancilla et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)
Characterization of Alzheimer's β-secretase protein BACE -: A pepsin family member with unusual properties
M Haniu et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2000)
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment
C Mateo et al.
ENZYME AND MICROBIAL TECHNOLOGY (2000)
Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability
R Fernandez-Lafuente et al.
ENZYME AND MICROBIAL TECHNOLOGY (2000)
Covalent immobilization of α-amylase onto poly(2-hydroxyethyl methacrylate) and poly(styrene-2-hydroxyethyl methacrylate) microspheres and the effect of Ca2+ ions on the enzyme activity
H Tümtürk et al.
FOOD CHEMISTRY (2000)
Encapsulation and biocatalytic activity of the enzyme pepsin in fatty lipid films by selective electrostatic interactions
A Gole et al.
CHEMICAL COMMUNICATIONS (2000)