Preparation and application of silver/chitosan-sepiolite materials with antimicrobial activities and low cytotoxicity

Antimicrobial materials can prevent microbial infection and affect the beauty and structure of interior walls. Herein, a hybrid material silver/chitosan-sepiolite (Ag/5CTs-Sep) with antimicrobial activities was prepared via impregnation. Its antimicrobial properties were investigated via the disk diffusion method. Results showed that the width of inhibition zone of Ag/5CTs-Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger reached 58.15, 32.95 and 35.18 mm, respectively. The Sep was a suitable carrier for increasing thermal stability and antimicrobial durability, and chitosan improved the dispersion of silver to enhance antimicrobial activities. In addition, characterization indicated that the modification of Sep by CTs can promote the formation of lattice oxygen in Ag/5CTs-Sep, which can induce a high reactive oxygen species (ROS) content, causing the death of microbials. The antifungal mechanism revealed that the death of Aspergillus niger was due to Ag/5CTs-Sep that induced the production of high ROS level and damaged cell membrane. Moreover, Ag/5CTsSep possessed low cytotoxicity, and an applied test of the water-based coatings showed that the addition of Ag/ 5CTs-Sep could both effectively inhibit microorganisms and meet the performance standards for water-based coatings. This work may provide new guidance for the design and application of antibacterial materials.

Automated summary

In this study, a hybrid material silver/chitosan-sepiolite (Ag/5CTs-Sep) with antimicrobial properties was successfully prepared. The material exhibited high inhibitory effects against Staphylococcus aureus, Escherichia coli, and Aspergillus niger. The antimicrobial mechanism was revealed through characterization, and the material showed low cytotoxicity. Moreover, the material was found to be suitable for water-based coatings and met the performance standards.