期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 202, 期 -, 页码 68-79出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.01.043
关键词
Polygonatum kingianum polysaccharides; Nephrotoxicity; HK-2 cell; Oxidative stress; Mitochondrial-mediated apoptosis; Preventive treatment
资金
- Sichuan Science and Technology Program [2019YFS0183]
- CAEP Foundation [CX20200017]
- National Natural Science Foundation of China [22006140, 21703214]
The study found that Polygonatum kingianum (PK) can effectively detoxify and prevent uranium-induced nephrotoxicity. Polysaccharides and aqueous extract of PK can enhance metabolic activity, alleviate cell damage and apoptosis. The study also found that PK alleviates uranium-induced cytotoxicity by regulating mitochondria-mediated apoptosis and the GSK-3 beta/Fyn/Nrf2 pathway.
The current detoxification options of uranium, a toxic radioactive heavy metal, have obvious side effects. Polygonatum kingianum (PK), a natural product with the function of antioxidant, may be effective in detoxification and prevention of uranium-induced nephrotoxicity. Here, we studied the protective effects of PK polysaccharides (PKP) and aqueous extract (PKAE) on uranium-induced toxicity in human kidney (HK-2) cells. First, the physicochemical properties of PKP and PKAE were characterized. Assays on cultured cells demonstrated that pretreatment with PKP and PKAE significantly increased metabolic activity, relieved morphological impairments, and alleviated apoptosis. The impairments caused by uranium exposure were ameliorated (mitochondrial membrane potential and ATP level increased while reactive oxygen species decreased). Molecular mechanistic studies revealed that PKP and PKAE alleviated uranium-induced cytotoxicity by regulating mitochondria mediated apoptosis and the GSK-3 beta/Fyn/Nrf2 pathway. Collectively, our data support the preventive and therapeutic applications of PKP and PKAE for uranium poisoning.
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