Multilayer dextran derivative based capsules fighting bacteria resistant to Antibiotic: Case of Kanamycin-Resistant Escherichia Coli

Bacteria resistance to antibiotics has emerged as a major health problem. Developing new antibacterial systems is then of major interest. In this sense, we present biocapsules presenting inherent antibacterial capacity. The self-assembly of charged biopolymer, namely diethylaminoethyl-dextran hydrochloride (dex(+)) and dextran sulfate (dex(-)), were done on calcium carbonate micmparticles, used as a template. Zeta potential measurements have shown the successful alternate adsorption of these biopolymers and related charge reversal upon the multilayer film construction onto the particles surface. The shape of the capsules was characterized by scanning electron microscopy (SEM). These particles were tested against bacteria resistant to antibiotics, namely kanamycin-resistant Escherichia coli. An inhibitory effect of the particles was observed during bacterial growth in liquid medium, i.e. in the range of 10 % for (dex(+)/dex(-))(n) coated CaCO3 materials and of 50% for (dex(+)/dex(-))(n )capsules. These findings evidence the high potential of capsules to act as antimicrobial agents in future and in treatments against infections.

Automated summary

Bacteria resistance to antibiotics is a major health problem, and developing new antibacterial systems is crucial. This study presents biocapsules with inherent antibacterial capacity, formed by self-assembly of charged biopolymers on calcium carbonate microparticles. The capsules showed significant inhibitory effects against antibiotic-resistant bacteria, indicating their potential as antimicrobial agents in future treatments against infections.