期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 203, 期 -, 页码 244-255出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.01.143
关键词
Quercetin; Hohenbuehelia serotina polysaccharides; Physicochemical characteristic; Gastrointestinal digestion; Anti-proliferative activity
资金
- National Natural Science Foundation of China [31972080]
- Hebei Provincial Department of Ed-ucation High School Science and Technology Plan Project [QN2019060]
- Hebei Provincial Introduction of Overseas Students Funding Project [C20200363]
- Natural Science Foundation of Hebei Province [C2019203484]
In this study, self-assembled nanoparticles based on Hohenbuehelia serotina polysaccharides were fabricated to encapsulate quercetin and improve its bioavailability. The nanoparticles exhibited excellent physicochemical stability and sustained-release characteristics during gastrointestinal digestion. After digestion, the nanoparticles showed significant anti-proliferative activity on HeLa cells, inducing cell cycle arrest and apoptosis through regulation of key proteins.
In this study, the self-assembled nanoparticles based on Hohenbuehelia serotina polysaccharides (QC-HSP NPs) were fabricated to encapsulate quercetin for improving its bioavailability. The structural characteristics, physicochemical properties as well as the cytotoxicity activities of QC-HSP NPs during gastrointestinal digestion in vitro were respectively investigated. The results showed that QC-HSP NPs possessed the spherical and smooth surface morphology, with the average particle size of 360 nm and zeta potential of-38.8 mV. Moreover, QC-HSP NPs had excellent physiochemical stabilities, and presented sustained-release characteristics during gastrointestinal digestion in vitro. Compared with undigested ones, QC-HSP NPs after gastrointestinal digestion exhibited the more significant anti-proliferative activity on HeLa cells through accumulation of intracellular ROS, arrest of cell cycle at G2/M phase by regulation of cyclin B1, CDK1, p53 and p21 and induction of apoptosis by ER apoptosis pathway. This study provides a new strategy for designing quercetin-loaded nanoparticles based on natural polysaccharides to improve the bioavailability of quercetin.
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