期刊
INSECT SCIENCE
卷 30, 期 1, 页码 95-108出版社
WILEY
DOI: 10.1111/1744-7917.13048
关键词
Diaphorina citri; insulin-like peptide; insulin receptor; insulin signaling; reproduction; target of rapamycin
类别
This study found that the expression levels of upstream factors DcILP1, DcILP2, and DcIR in the insulin signaling system of Diaphorina citri are upregulated after feeding on young shoots, which in turn influences the expression of the upstream regulatory gene DcRheb of the TOR signaling pathway and eventually leads to the maturation of the ovaries and oviposition behavior of female D. citri.
Diaphorina citri is an important vector of Citrus Huanglongbing (HLB) disease. After feeding on young host plant shoots, the population of D. citri can increase significantly. Females also only lay eggs on young shoots. However, there are few studies on the mechanism of this phenomenon. Exogenous nutrient signals can affect the insulin signaling system of D. citri after feeding on young shoots. In this study, the expression of upstream factors DcILP1, DcILP2, and DcIR in the insulin signaling system of D. citri was upregulated after feeding on young shoots. After being silenced by RNA interference technology, the results showed that the number of oviposited eggs of D. citri was significantly decreased and the ovarian development was inhibited with severe vacuolation. In addition, detection using quantitative reverse transcription-polymerase chain reaction showed that the upstream regulatory gene DcRheb of the target of rapamycin (TOR) pathway and the downstream reproduction-related DcVg gene were also significantly downregulated. These results suggest that feeding upon young shoots may upregulate the expression levels of upstream factors DcILP1, DcILP2, and DcIR in the insulin signaling system. The signal will be through upregulating the expression of DcRheb, an upstream gene of the TOR signaling pathway. This in turn influences yolk metabolism, which eventually causes the ovaries of female D. citri to mature and therefore initiate oviposition behavior.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据