期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 177, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2022.114519
关键词
Rockrose; Hemicellulosic hydrolysates; Detoxification; Glucan-rich solids; Enzymatic saccharification; Lactic acid
资金
- project SelecTEcoli from the Operational Program Alentejo (Alentejo 2020) -Support System for Scientific Research-Projects for Scientific Research and Technological Development (IC & DT) through the European Regional Development Fund (ERDF) [ALT20-03-0145-FEDER-000034]
- Universidad Nacional Autonoma de Mexico [PAPIIT-DGAPA IV100119]
- Operational Programme for Competitiveness and Internationalization (PORTUGAL 2020) [BBRI-LISBOA-01-0145-FEDER-022059]
- Lisbon Portugal Regional Operational Programme (Lisboa 2020)
- North Portugal Regional Operational Programme (Norte 2020) under the Portugal 2020 Partnership Agreement, through the ERDF
- CAPES Foundation
- Ministry of Education of Brazil, Brasilia [DF 700 40-020, 9109/13-7]
- Fundacao para a Ciencia e Tecnologia [UID/AGR/00239/2020, UIDB//05183/2020]
In this study, glucan-rich solids and xylose-rich hydrolysates from Cistus ladanifer distillery residues were used for D-lactic acid production. The hydrolysates were subjected to post-hydrolysis under optimized conditions, and subsequent detoxification procedures were performed. The results showed that both hemicellulose- and cellulose-derived sugars can efficiently be used as carbon sources for D-lactic acid production. The study provides insights into optimizing the production of D-lactic acid from distillery residues.
In this study, glucan-rich solids, and xylose-rich hydrolysates obtained from Cistus ladanifer distillery residues (CLR) were used for D-lactic acid (D-LA) production by the D-lactogenic Escherichia coli strain JU15. Firstly, hemicellulosic hydrolysates obtained by the autohydrolysis process were submitted to dilute sulfuric acid catalysed post-hydrolysis. The influence of operational conditions on oligosaccharides hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.1-2.3. The optimum post-hydrolysis conditions were found for CS of 1.6 (300 mM H2SO4, 15 min, 121 degrees C). Subsequent detoxification procedures on post hydrolysed liquors were carried out, where 9.1% (w/v) powdered activated charcoal enabled a full removal of furfural, 5-hydroxymethylfurfural (HMF), and phenolic compounds together with a reduction of acetic acid (37%), and formic acid (27%). Diverse fermentation modes using detoxified and non-detoxified hydrolysates, as well using previously NaOH delignified glucan-rich solids alone (SHF or SSF) or together with pentoses liquors (SSCF) (5% loading) were performed. For all the tested conditions, both hemicellulose-and cellulose-derived sugars can be efficiently used as the carbon source to produce D-lactic acid by E. coli JU15 with a D-LA yield always surpassing 92 g(D-LA)/100 g sugars.
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