期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 177, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2021.114468
关键词
Annona squamosa; Oligosaccharide; Structure analysis; Inhibition on alpha-glucosidase; Molecular docking
资金
- National Natural Science Foundation of China [31871791]
- Graduate Research and Innovation Project of Tianjin [2019YJSB008]
- Open Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science Technology [SKLFNS-KF-202017]
This study utilized pectinase to degrade Annona squamosa polysaccharide (ASP) and obtained a novel oligosaccharide Z. The structure of Z was identified and its inhibitory activity against α-glucosidase was demonstrated. The oligosaccharide Z has the potential to be utilized as an alpha-glucosidase inhibitor.
Annona squamosa (AS) has relatively high content of polysaccharides which had effective hypoglycemic activity. The oligosaccharides have advantages in the digestion and absorption. However, there were few reports on oligosaccharides from AS. In the manuscript, pectinase was utilized to degrade Annona squamosa polysaccharide (ASP). The product was acetylated, purified by silica gel column and deacetylated to obtain a novel oligosaccharide Z. The structure of Z was identified with NMR, mass spectrometry and monosaccharide composition analysis. The results demonstrated that the oligosaccharide Z was a trisaccharide with molecular weight of 518 Da. The structure of oligosaccharide Z was linear skeleton composed of beta-D-Glc-(1 -> 2)-beta-D-Glc-(1 -> 3)-alpha-D-GalA. The oligosaccharide Z can effectively inhibit the activity of a-glucosidase with reversible type and mixed-type inhibition. The oligosaccharide Z had effective binding effect with alpha-glucosidase from molecular docking experiments. The results revealed that the oligosaccharide Z has the potential to be utilized as alpha-glucosidase inhibitor. The manuscript provided theoretical basis for the new applications of AS in food industry and pharmaceutical industry. It also provided valuable clues for finding new potential hypoglycemic drugs from natural sources.
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