4.7 Article

Development of Continuous Flow Analysis System Based on Amperometric Biosensors

期刊

IEEE SENSORS JOURNAL
卷 22, 期 8, 页码 7542-7549

出版社

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSEN.2022.3156660

关键词

Biosensors; Electrodes; Fluidics; Pumps; Valves; Current measurement; Ethanol; Amperometric biosensors; amperometric detection; continuous flow analysis (CFA); potentiostat

资金

  1. Spanish Government (Inter-Ministerial Commission of Science and Technology) [TRACE2009_0267_02]

向作者/读者索取更多资源

This article introduces an automated CFA system based on amperometric biosensors. The system possesses characteristics of fast response time, practicality, and low-cost equipment, and is suitable for real-time monitoring and automation. The study successfully designs and develops all parts of the system, and validates the prototype using an amperometric biosensor to analyze the ethanol content of low-alcohol beers.
Although the basic theory for electrochemical measurement is well known, there is still much to be developed in specific instrumental systems for biosensors, especially to achieve fast response times, usefulness, and low-cost equipment. In this regard, continuous flow analysis (CFA) systems with an integrated biosensor are valuable tools for bioanalysis monitoring. These systems are suitable for automation and monitoring in real time with a short response time. Among biosensors, amperometric ones are the most used due to their high selectivity and wide linear range. In this work, we design and develop all parts of an automated CFA system for bioanalysis based on amperometric biosensors. It consists of several inlets for the samples, standards, and carrier solution; a fluidic subsystem; an electronic subsystem in which output voltage will be directly correlated to the analyte concentration in the solution; and a software application to configure and control all the features of the system. The prototype was successfully validated using an amperometric biosensor to analyze the ethanol content of low-alcohol beers.

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