期刊
GENOMICS
卷 114, 期 2, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2022.01.002
关键词
Hepatocellular carcinoma; N6AMT1; ALKBH1; N6-methyladenine; Genomic distribution
资金
- National Natural Science Foundation of China [81172193, 82072035]
The expression of N6AMT1 and ALKBH1 in hepatocellular carcinoma (HCC) cells affects cell viability, apoptosis, migration, and invasion. Differential gain and loss of 6mA regions were identified in HCC tissues, mainly in intergenic regions. Some differential 6mA modifications were found to be associated with tumors, oncogenes, and tumor suppressor genes.
DNA N6-methyladenine (6 mA) is a new type of DNA methylation identified in various eukaryotic cells. However, its alteration and genomic distribution features in hepatocellular carcinoma (HCC) remain elusive. In this study, we found that N6AMT1 overexpression increased HCC cell viability, suppressed apoptosis, and enhanced migration and invasion, whereas ALKBH1 overexpression induced the opposite effects. Further, 23,779 gain-of-6 mA regions and 11,240 loss-of-6 mA regions were differentially identified in HCC tissues. The differential gain and loss of 6 mA regions were considerably enriched in intergenic regions. Moreover, 7% of the differential 6 mA modifications were associated with tumors, with 60 associated with oncogenes and 57 with tumor suppressor genes (TSGs), and 17 were common to oncogenes and TSGs. The candidate genes affected by 6 mA were filtered by gene ontology (GO) and RNA-seq. Using quantitative polymerase chain reaction (qPCR), BCL2 and PARTICL were found to be correlated with DNA 6 mA in certain HCC processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据