期刊
GENOME
卷 65, 期 7, 页码 363-375出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2021-0080
关键词
Gentianopsis barbata; chloroplast genome; phylogenetic relationship
资金
- National Natural Science Foundation of China [U1812403-1, 82073960]
- Chengdu University of Traditional Chinese Medicine [003109034001]
- Beijing Natural Scientific Foundation [7202135]
- National Science & Technology Fundamental Resources Investigation Program of China [2018FY100701]
This study sequenced and characterized the complete chloroplast genome of Gentianopsis barbata, an essential medicinal plant in China. The results provide valuable insights into plastome evolution and important information on the genetic structure and diversity of G. barbata.
Gentianopsis barbata is an essential medicinal plant in China with high ornamental and medicinal values. Unfortunately, the study of the chloroplast genome of this plant still has a gap. This study sequenced and characterized the complete chloroplast genome of G. barbata. The complete chloroplast genome of G. barbata is a typical circular structure of 151 123 bp. It consists of a large single-copy region (82 690 bp) and a small single-copy region (17 887 bp) separated by a pair of inverted repeats (25 273 bp), which covers 78 protein-coding genes, 30 tRNAs, and 4 rRNAs. The long repeat sequence analysis showed that the P-type (palindromic) sequences were the major long repeat sequences. Thirty-seven simple sequence repeats were identified, most of which were single nucleotides. The Bayesian inference tree, maximum likelihood tree, and neighbor-joining tree suggested that G. barbata is grouped with Gentianopsis grandis and Gentianopsis paludosa. The divergence time analysis showed that G. barbata diverged at 1.243 Mya. Comparative analysis of chloroplast genomes can reveal interspecific diversity, and regions with high variation can be used to develop molecular markers applicable to various research areas. Our results provide a new insight into plastome evolution and a valuable resource for further studies on G. barbata.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据