4.6 Article

IQGAP1 enhances cell invasion and matrix metalloproteinase-2 expression through upregulating NF-kappa B activity in esophageal squamous cell carcinoma cells

期刊

GENE
卷 824, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.gene.2022.146406

关键词

IQGAP1; MMP2; Invasion; NF-kappa B

资金

  1. Natural Science Foundation of Shanxi Province, China [201901D111214]
  2. Key Laboratory of Cellular Physiology (Shanxi Medical University) , Ministry of Education, China [KLMC/SXMU-201906]

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This study found that IQGAP1 is highly expressed in esophageal squamous cell carcinoma (ESCC) and is positively correlated with MMP2 expression. IQGAP1 overexpression promotes the growth, migration, and invasion of ESCC cells, upregulates MMP2 expression, and increases NF-kappa B expression and nuclear localization. Treatment with PDTC or JSH-23 can reverse IQGAP1-mediated cell migration and invasion ability, as well as MMP2 expression.
Esophageal squamous cell carcinoma (ESCC) is one type of the most common malignancies, yet the overall survival rate is still not ideal. IQ motif containing GTPase activating protein 1 (IQGAP1) participates in cell biological functions of various tumors as an oncogene. However, the mechanisms of IQGAP1 affecting malignant development of ESCC are still unclear. In this study, the expression and correlation of IQGAP1 and MMP2 in esophageal cancer tissues were evaluated by online databases and immunohistochemistry. Stably transfected cell lines with IQGAP1 overexpression and knockdown were constructed. Cell growth, migration and invasion ability, the expression of MMP2 and NF-kappa B expression were examined in ESCC cells. Furthermore, the cellular malignant phenotypes of ESCC and MMP2 expression in IQGAP1 overexpressing cells after treatment with the NF-kappa B inhibitor pyrrolidinecarbodithioic acid (PDTC) or JSH-23 were detected. We found that the expression of IQGAP1 and MMP2 were up-regulated and positively correlated in ESCC tissues. IQGAP1 overexpression promoted the growth, migration and invasion of ESCC cells, and up-regulated the expression of MMP2, and increased the expression and the nuclear localization level of NF-kappa B. Treating with PDTC or JSH-23 reversed IQGAP1-mediated cell migration and invasion ability, as well as the expression of MMP2. In summary, IQGAP1 plays a tumor promotion role to regulate the migration and invasion of ESCC cells and the expression of MMP2 through upregulating NF-kappa B activity, supporting a promising therapeutic target against ESCC.

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