An acidic polysaccharide from Patinopecten yessoensis skirt prevents obesity and improves gut microbiota and metabolism of mice induced by high-fat diet

The prevention of obesity is an urgent need for worldwide public health. The scallop (Patinopecten yessoensis) skirt is the by-product of adductor processing. In order to explore the value of the scallop skirt, the present study isolated an acidic polysaccharide from the skirt of P. yessoensis (SPYP) and evaluated its anti-obesity effect. SPYP was characterized as an acidic heteropolysaccharide with a molecular weight of 13.58 kDa. Through stepwise acid hydrolysis followed by HPLC-MSn analysis, two core chains in SPYP was determined as heparin-like--> 4)beta-GlcA(1 -> 4)alpha-GlcNAc- -> and chondroitin sulfate-like -> 4)beta-GlcA(1 -> 3)beta-GalNAc ->. SPYP could effectively reduce body weight, decrease fat accumulation and prevent high blood lipids induced by a high-fat diet (HFD) in mice. Further 16S ribosomal ribonucleic acid (rRNA) sequencing analysis revealed that the anti-obesity effect of SPYP was notably associated with its modulation on gut microbiota. The short-chain fatty acids were also restored by SPYP supplementation compared to the HFD group. Moreover, liquid chromatography-high resolution accurate mass spectrometry (LC-HRMS) analysis demonstrated that metabolite profiles of HFD-fed mice were altered by SPYP supplementation, especially the lipid metabolism in serum and amino acid metabolism in cecal content, which may contribute to the anti-obesity effect of SPYP. The present study offers the basis for processing and application of P. yessoensis skirt.

Automated summary

The study isolated an acidic polysaccharide (SPYP) from the skirt of Patinopecten yessoensis and found that it has anti-obesity effects by reducing body weight, fat accumulation, and high blood lipids induced by a high-fat diet in mice. The modulation of gut microbiota and restoration of short-chain fatty acids by SPYP supplementation were key factors in its anti-obesity effect. Liquid chromatography-high resolution accurate mass spectrometry analysis showed that SPYP altered metabolite profiles in HFD-fed mice, particularly affecting lipid and amino acid metabolism.