Monitoring pH and whey protein digestion by TD-NMR and MRI in a novel semi-dynamic in vitro gastric simulator (MR-GAS)

Gastric digestion is crucial for protein breakdown. Magnetic resonance techniques have a great deal of potential but remain underexplored with regard to their application in the study of food digestion via MRI-markers, such as transverse (R-2) and longitudinal (R-1) relaxation rates. R-2 has been used to monitor gastric digestion of whey protein gels, but only in a static in vitro model. It is essential to investigate whether relaxation rates can be valid measures of digestion under dynamic circumstances. We developed a novel MRI-compatible semi-dynamic gastric simulator (MR-GAS) that includes controlled gastric secretion, emptying and mixing at body temperature. PH and protein hydrolysis were measured during protein gel digestion in the MR-GAS. R-2 and R-1 of the supernatant were measured by time-domain nuclear magnetic resonance (TD-NMR). The stomach chamber of the MR-GAS was also scanned with MRI to measure R-2 and R-1. For TD-NMR, 99% of the variance in R-2 and 96% of variance in R-1 could be explained as a function of protein concentration and [H+]. For MRI, the explained variances were 99% for R-2 and 60% for R-1. From these analysis, the obtained equations enabled the prediction of protein concentration and pH by R-2 and R-1. The normalised root mean squared deviation of the predictions for protein concentration were 0.15 (NMR) and 0.18 (MRI), and for pH were 0.12 (NMR) and 0.29 (MRI). In conclusion, the MR-GAS model may be used in a clinical MRI to monitor gastric digestion under in vitro dynamic circumstances, by measuring R-2 and R-1. These results underscore the potential of MRI to monitor nutrients hydrolysis and pH changes in future in vivo studies.

Automated summary

Gastric digestion is essential for protein breakdown and magnetic resonance techniques show potential in studying this process. A novel MRI-compatible semi-dynamic gastric simulator was developed to monitor digestion under dynamic circumstances, showing high potential for future in vivo studies in monitoring nutrient hydrolysis and pH changes.