期刊
FOOD CONTROL
卷 133, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2021.108669
关键词
Seafood; Escherichia coli; Pathogroup; Fecal contamination; Risk assessment; Serotype
资金
- ICAR-Central Institute of Fisheries Education
Seafood contamination with pathogenic E. coli poses a significant public health threat in developing countries. This study identified various pathogroups of E. coli in fresh seafood samples, with 55 out of 78 samples contaminated with pathogenic E. coli. The use of TP broth enrichment and SMAC agar isolation appeared to be the most effective method for recovery of pathogenic E. coli from seafood.
Seafood contamination with pathogenic Escherichia coli is a significant public health threat in developing countries. In this study, the prevalence of different pathogroups of E. coli was investigated in fresh seafood. A total of 78 samples comprising of finfish (41) and shellfish (37) were analyzed using multiple selectiveenrichment-isolation protocols. From all samples, 639 E. coli were isolated and tested for the presence of pathogroup-specific virulence-associated genes by polymerase chain reaction (PCR). Based on the distribution of virulence genes, 118 isolates belonged to enterohemorrhagic E. coli (EHEC) group, 25 to enteropathogenic E. coli (EPEC), 20 to enterotoxigenic E. coli (ETEC), 16 to enteroaggregative E. coli (EAEC) and 1 to enteroinvasive E. coli (EIEC). Fifty-five samples of seafood, 29 finfish and 26 shellfish, were found contaminated with pathogenic E. coli. Among different enrichment broth-selective agar combinations used for the recovery of pathogenic E. coli from seafood, enrichment in Tryptone Phosphate (TP) broth followed by isolation on sorbitol MacConkey (SMAC) agar performed superior to others. The study highlights the importance of using selective isolation and genotyping protocols to identify human pathogenic E. coli in seafood in regions with extensive anthropogenic contamination.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据