期刊
FOOD CHEMISTRY
卷 377, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131931
关键词
Amantadine; Fluorescence quantitative; immunochromatographic assays; Background fluorescence quenching; immunochromatographic assay; Time-resolved immunoassay
资金
- National Key Research and Development Program of China [2019YFC1605500, 2018YFC1603500]
Background fluorescence quenching immunochromatographic assay (bFQICA) and time-resolved fluorescent immunochromatographic assay (TRFICA) have increasingly played a significant role in rapid detection technology for food safety. These assays provide rapid, precise, and reliable technical support for large-scale on-site screening and offer useful suggestions for monitoring harmful compounds.
The two sensitive fluorescence quantitative immunochromatographic assays (FQICAs), background fluorescence quenching immunochromatographic assay (bFQICA) and time-resolved fluorescent immunochromatographic assay (TRFICA), play an important role increasingly in rapid detection technology for food safety. Amantadine (AMD), used extensively in virus infections in livestock and poultry, has been prohibited due to hazard concerns over public human health. Therefore, AMD was used as a model molecule in the FQICAs establishment and comparison based on the same bioreagents. The outstanding performance in technical parameters of the two FQICAs indicated that they could provide rapid, precise, reliable technical support for large-scale on-site screening for AMD detection. What's more, the systematic and comprehensive comparison of the two FQICAs would give useful suggestions for scientists and users in monitoring the harmful compounds.
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