期刊
FOOD CHEMISTRY
卷 378, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132093
关键词
Foodborne pathogen; Microfluidic immunosensor; Stir bar enrichment; Copper-polydopamine nanoparticles; DNAzyme; Click reaction
资金
- National Natural Science Foundation of China [21974074, 51608479]
- Natural Science Foundation of Zhejiang Province [GDKLEEH201807, LY19B050001, 202002N3112, LY20B050004, LGC19B070003, LGN18H300001, LGC20B070001]
- K.C. Wong Magna Fund in Ningbo University
- Natural Science Foundation of Ningbo [GDKLEEH201807, LY19B050001, 202002N3112, LY20B050004, LGC19B070003, LGN18H300001, LGC20B070001]
This study developed a microfluidic immunosensor for ultrasensitive detection of Staphylococcus aureus (S. aureus) using stir bar enrichment and DNAzyme-assisted click reaction. The method showed excellent sensitivity and simplicity, with satisfactory results in detecting real samples.
This work demonstrated an ultrasensitive and simple microfluidic immunosensor for point-of-care test of Staphylococcus aureus (S. aureus) based on the stir bar enrichment and DNAzyme-assisted click reaction. Initially, S. aureus was enriched by the 4-mercaptophenylboronic acid-functionalized stir bar. The yolk antibody (immunoglobulin Y) and copper-labeled polydopamine nanoparticles were then specifically conjugated with the captured target. The Cu(II) was released under acidic conditions and effectively catalyzed the copper-catalyzed azide-alkyne cycloaddition (CuAAC) between the alkyne group-labeled DNAzyme and the streptavidin-biotinazido with the assistance of DNAzyme. Finally, the DNAzyme-streptavidin complexes were detected by microfluidic chips to quantify S. aureus. Under optimum conditions, this immunosensor showed good detection performances toward S. aureus within 10 to 2.5 x 104 CFU/mL with a limit of detection of 3 CFU/mL. Moreover, the satisfying detection results of real samples of animal origin also implied that this immunosensor owned great potential in practical applications.
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