Oral retention of thermally denatured whey protein: In vivo measurement and structural observations by CD and NMR

This study investigated structural changes and the in vivo retention in the oral cavity of heated whey protein concentrate (WPC). Heated WPC was shown to have both a higher retention time in the oral cavity compared to unheated whey protein up to 1 min post swallow, and a concomitant increase in free thiol concentration. Nuclear magnetic resonance and circular dichroism demonstrated structural changes in the secondary and tertiary structures of the WPC upon heating. Structural loss of the beta-barrel was shown to increase during heating, leading to the exposure of hydrophobic regions. The increase in free thiols and hydrophobic regions are two factors which are known to increase mucoadhesive strength and hence increase oral retention of heated whey protein which may subsequently increase the perception of mouthdrying.

Automated summary

This study found that heated whey protein concentrate has a longer retention time in the oral cavity compared to unheated WPC, causing structural changes, increased free thiol concentration, and exposure of hydrophobic regions. These factors contribute to increased mucoadhesive strength and mouthdrying perception.