4.7 Article

Click reaction triggered turn-on fluorescence strategy for highly sensitive and selective determination of steroid hormones in food samples

期刊

FOOD CHEMISTRY
卷 374, 期 -, 页码 -

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ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131565

关键词

Steroid hormones; CuAAC; Fluorescence derivatization; Food samples

资金

  1. National Natural Science Foundation of China [22076115, 32022069]
  2. Youth Innovation Team of Shaanxi Universities [21JP020]

向作者/读者索取更多资源

A promising click fluorescent labeling method with high selectivity for the determination of five steroid hormones in food samples was proposed. The method relies on the reaction between 3-Azido-7-hydroxycoumarin and the alkynyl group of steroid hormones in the presence of copper (I), resulting in a turn-on fluorescence response. The method showed excellent performance with good linearity and low detection limit when detected by HPLC-FLD.
The copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction has becoming noticeable in the field of analytical chemistry. Mild reaction conditions, simple operation, high efficiency, and good regioselectivity make this classical click reaction a perfect strategy for chemical derivatization. Herein, we proposed a promising click fluorescent labeling method with high selectivity for the determination of five steroid hormones in food samples. The labeling strategy depends on the reaction between 3-Azido-7-hydroxycoumarin and the alkynyl group of steroid hormones, which shows a turn-on fluorescence response in the presence of copper (I). The formed fluorescent products were detected by HPLC-FLD. Under the optimized conditions, the proposed method presented excellent performance with good linearity (R-2 >= 0.9998) and low detection limit (1.8-7.3 mu g L-1). Further, satisfactory recoveries were obtained to be 82-107% in spiked meats with relative standard deviations (RSDs) <= 5.7%. Finally, the established method was successfully applied for the determination of steroid hormones in meat, indicating the potential prospect of the click reaction in chemical derivatization.

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