期刊
FOOD CHEMISTRY
卷 371, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131075
关键词
Peptide markers; Sample preparation; Meat authentication; LC-MS; MS
资金
- National Natural Science Foundation of China - Ministry of Science and Tech-nology of the People's Republic of China [2017YFC1601704]
This study introduced a rapid sample preparation method, established a rapid LC-MS/MS method for meat species identification, successfully screened 35 species-specific peptides, detected the meat species of 20 processed meat products, and found potentially adulterated samples.
The adulteration of meat products has been reported worldwide, and detection of specific peptides through mass spectrometry (MS) is a reliable method for meat species identification. However, the practical application of this method is limited by complicated steps and long reaction time of the traditional sample preparation. Therefore, this paper introduced a convenient and time-saving sample preparation by optimizing the steps of reduction, alkylation, digestion, and purification. With the rapid sample preparation, 35 species-specific peptides for seven species (pig, cattle, sheep, deer, chicken, duck, and turkey) were screened using high-resolution MS, and a rapid LC-MS/MS method was established. The method only takes 3 h from sample receipt to results. The meat species of 20 processed meat products were detected, and three samples were found potentially adulterated. The method is proved to have high sensitivity, specificity, practicability with respect to rapid identification of meat species in meat products.
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