期刊
FOOD CHEMISTRY
卷 373, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.131647
关键词
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资金
- Special Basic Research Fund for Central Public Research Institutes [2019-YWF-YB-01, Y2020PT19]
- National Natural Science Foundation of China [31802073]
- Chinese Academy of Agricultural Science and Technology Innovation Project [ASTIP-IAS-12]
- Special R&D Program Project of the Chinese Academy of Se-enriched Industry [2019QCY-3.2, 2019QCY-3.3]
- Key Laboratory of Se-Enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Laboratory of Se-Enriched Food Development [Se-2018HZ01]
Nap-DNB, an off-on fluorescent probe based on naphthimide, was developed for the detection of biological selenols in vitro. It can rapidly and sensitively respond to selenocysteine, with a detection limit of 92 nM. By adopting a low-pH detection environment and robust reaction sites, Nap-DNB successfully detected selenols in selenium-enriched Tan sheep's serum, liver, and longissimus dorsi.
An off-on fluorescent probe, Nap-DNB, which is based on naphthimide, was designed and developed for the detection of biological selenols in vitro. We have adopted a combination of a low-pH detection environment and reaction sites that are more difficult to destroy to avoid the interference of a large number of biological thiols in biological samples. Nap-DNB can completely respond to selenocysteine within 15 mins, with a detection limit of 92 nM. Nap-DNB was successfully used for the detection of selenols in the serum, liver, and longissimus dorsi of selenium-enriched Tan sheep. Through comparison, we found that the detection of selenols by the Nap-DNB is similar to that by thioredoxin reductase and glutathione peroxidase in a commercial kit method. Nap-DNB can be used for the detection of selenols in selenium-enriched Tan sheep.
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