Effect of solvent type on ultrasound-assisted extraction of antioxidant compounds from Ficaria kochii: Optimization by response surface methodology

The optimization of extraction conditions to recover antioxidant rich extract from plant of Ficaria Kochii with an ultrasound-assisted system was achieved by response surface methodology. The rotatable central composite design (RCCD) was used to optimize extraction parameters in terms of total phenolic and total flavonoid content and antioxidant activity. The optimum conditions for extraction by methanol were: ratio of solvent to raw material, 10%; extraction time, 50 min and extraction temperature, 60 degrees C. Under these conditions, the obtained total phenolic content 46.379 mg gallic acid equivalents/g dry matter and total flavonoid content 11.754 mg quercetin equivalents/g dry matter with antioxidant property (DPPH: the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity) was 89.548%. Also, the optimum operating conditions for extraction by water were: ratio of solvent to raw material, 10%; extraction time, 30 min and extraction temperature, 40 degrees C. Under these conditions, the obtained total phenolic content 51.112 mg gallic acid equivalents/g dry matter and total flavonoid content 4.331 mg quercetin equivalents/g dry matter with antioxidant property was 97.004%. This study suggests that the antioxidant rich extract from plant of Ficaria Kochii can be a candidate for food antioxidant and Due to these features, this extract can be used in food storage.

Automated summary

The optimization of extraction conditions for the antioxidant-rich extract from Ficaria Kochii plant was achieved using an ultrasound-assisted system and response surface methodology. The study found that the optimal conditions for extraction using methanol were a solvent-to-raw material ratio of 10%, an extraction time of 50 minutes, and an extraction temperature of 60 degrees C. Under these conditions, the extract had a total phenolic content of 46.379 mg gallic acid equivalents/g dry matter, a total flavonoid content of 11.754 mg quercetin equivalents/g dry matter, and an antioxidant activity of 89.548%. Similarly, the optimal conditions for extraction using water were a solvent-to-raw material ratio of 10%, an extraction time of 30 minutes, and an extraction temperature of 40 degrees C. The resulting extract had a total phenolic content of 51.112 mg gallic acid equivalents/g dry matter, a total flavonoid content of 4.331 mg quercetin equivalents/g dry matter, and an antioxidant activity of 97.004%. This study suggests that the antioxidant-rich extract from Ficaria Kochii could be used as a candidate for food antioxidants and for food storage.