4.2 Review

Bacteriophage therapy in aquaculture: current status and future challenges

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An Appraisal of Bacteriophage Isolation Techniques from Environment

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Summary: Researchers have shown renewed interest in bacteriophages due to their potential role in various applications and as natural killers of bacteria. With rising antimicrobial resistance, bacteriophages are increasingly sought for therapy, leading to a focus on isolation and characterization techniques. The review also highlights the need for further development of on-field bacteriophage isolation techniques.

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Summary: This review explores the composition and evolution of phage communities and their roles in controlling the population and evolutionary dynamics of bacterial communities. The study emphasizes the need for greater ecological realism in laboratory studies to better understand the complexity of microbial communities thriving in natural environments.

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Characterization and protective effects of lytic bacteriophage pAh6.2TG against a pathogenic multidrug-resistant Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus)

Le Thanh Dien et al.

Summary: This study identified a new lytic phage, pAh6.2TG, which showed high specificity and stability against multidrug-resistant Aeromonas hydrophila. In experimental challenges, phage treatment significantly improved survivability of Nile tilapia exposed to the pathogenic MDR A. hydrophila, while reducing bacterial concentrations in both water and fish body. These findings suggest that pAh6.2TG is an effective alternative to antibiotics for controlling MDR A. hydrophila in tilapia and possibly other freshwater fish species.

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Optimization of wall material for phage encapsulation via freeze-drying and antimicrobial efficacy of microencapsulated phage againstSalmonella

Kantiya Petsong et al.

Summary: This study optimized the wall materials for microencapsulated phage and found that the combination of WPI and trehalose at a 3:1 ratio had the highest encapsulation efficiency and stability. The encapsulated phage showed over 90% survivability under various pH conditions.

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Review Microbiology

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William H. Lewis et al.

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NATURE REVIEWS MICROBIOLOGY (2021)

Article Fisheries

Siphoviridae phage PH669 capable of lysing some strains of O3 and O4 serotypes in Vibrio parahaemolyticus

Zimeng Hu et al.

Summary: Phage PH669 was isolated from domestic sewage and shown to have a broad host range, including various serotypes of V. parahaemolyticus. It exhibits stability in a wide range of temperatures and pH levels, making it suitable for different environments. Additionally, PH669 can effectively inhibit host bacteria growth within 30 minutes of infection and belongs to siphoviridae based on morphology.

AQUACULTURE (2021)

Article Fisheries

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Article Fisheries

An inactivated bivalent vaccine effectively protects turbot (Scophthalmus maximus) against Vibrio anguillarum and Vibrio harveyi infection

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Zelong Zhao

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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021)

Article Microbiology

Bacteriophage-resistant Acinetobacter baumannii are resensitized to antimicrobials

Fernando Gordillo Altamirano et al.

Summary: The study found that the bacterial capsule of Acinetobacter baumannii serves as the receptor for the phages phi FG02 and phi CO01. Mutants resistant to the phages had mutations in genes responsible for capsule biosynthesis, leading to loss of capsule and disruption of phage adsorption. These phage-resistant strains showed decreased susceptibility to human complement, beta-lactam antibiotics, and alternative phages, and exhibited reduced fitness in vivo. Furthermore, phage therapy was effective in treating A. baumannii infection in a mouse model.

NATURE MICROBIOLOGY (2021)

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Shotgun metagenomic analysis reveals the prevalence of antibiotic resistance genes and mobile genetic elements in full scale hospital wastewater treatment plants

Ranjith Kumar Manoharan et al.

Summary: Wastewater treatment plants are hotspots of emerging antimicrobial genes and mobile genetic elements. Hospital wastewater influences the abundance of ARGs and MGEs in activated sludge.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2021)

Review Fisheries

In vitro antagonistic effect and in vivo protective efficacy of Gram-positive probiotics versus Gram-negative bacterial pathogens in finfish and shellfish

Hien Van Doan et al.

Summary: Gram-positive probiotic bacteria have been shown to be effective in preventing disease outbreaks in aquaculture by producing bioactive compounds that can inhibit potential pathogenic Gram-negative bacteria. They can also enhance host immune-physiological functions and improve disease resistance. Further research is needed to optimize probiotic dosages and understand the in vivo effects of Gram-positive probiotics.

AQUACULTURE (2021)

Article Microbiology

Bacteriophage-encoded enzymes destroying bacterial cell membranes and walls, and their potential use as antimicrobial agents

Lukasz Grabowski et al.

Summary: The emergence of pathogenic bacteria resistant to most, if not all, known antibiotics is a significant medical issue. Developing novel antibacterial therapies, such as using bacteriophage-encoded enzymes, is crucial for future efficient treatment of bacterial infections. Understanding the biological functions and properties of these enzymes is essential for their potential use as antibacterial agents.

MICROBIOLOGICAL RESEARCH (2021)

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Francesca Micoli et al.

Summary: The use of antibiotics has successfully treated bacterial infections, but the emergence of antimicrobial resistance is a global threat. Vaccines used prophylactically can play a major role in fighting antimicrobial resistance by reducing infectious disease cases and antibiotic use.

NATURE REVIEWS MICROBIOLOGY (2021)

Article Microbiology

Phage-Mediated Control of Flavobacterium psychrophilum in Aquaculture: In vivo Experiments to Compare Delivery Methods

Valentina Laura Donati et al.

Summary: Phage-based methods have potential as sustainable alternatives to antibiotics in aquaculture, with oral administration showing promise in delivering phages to fish organs. However, higher phage dosages may be needed to effectively protect fish against Flavobacterium psychrophilum infections.

FRONTIERS IN MICROBIOLOGY (2021)

Article Microbiology

Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

Chia Wanq Tan et al.

Summary: This study isolated six bacteriophages from seafood samples that specifically infect Vibrio parahaemolyticus strains. The bacteriophages exhibited diverse morphological features and genetic characteristics, with a narrow host range specificity. Results showed that the bacteriophages were stable over a wide range of temperatures and pH levels, and a bacteriophage cocktail was more efficient in controlling the growth of Vibrio parahaemolyticus compared to a single bacteriophage suspension.

FRONTIERS IN MICROBIOLOGY (2021)

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Application of bacteriophage-derived endolysins to combat streptococcal disease: current State and perspectives

Sara B. Linden et al.

Summary: The decline in new antibiotic candidates and the increase in antibiotic resistance have led to the development of alternative antimicrobials, such as bacteriophage-encoded endolysins. Current studies focus on the discovery, structure/function, engineering, immunogenicity, toxicity/safety, and efficacy of endolysins, with some already in human clinical trials. Streptococcal diseases primarily affect accessible mucous membranes, making them a promising target for protein therapeutics, such as targeted endolysin therapy.

CURRENT OPINION IN BIOTECHNOLOGY (2021)

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Protective efficacy of phage PVN02 against haemorrhagic septicaemia in striped catfish Pangasianodon hypophthalmus via oral administration

Thi Hoang Oanh Dang et al.

Summary: This study investigated the protective efficacy of bacteriophage PVN02 against haemorrhagic septicaemia in striped catfish through oral administration. The results showed that this method could be a practical way to prevent disease in large-scale striped catfish farms.

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Synergistic action of phage phiIPLA-RODI and lytic protein CHAPSH3b: a combination strategy to target Staphylococcus aureus biofilms

Ana Catarina Duarte et al.

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A novel vibriophage vB_VhaS_PcB-1G capable of inhibiting virulent Vibrio harveyi pathogen

Liting Wu et al.

Summary: The study investigated the phenotypic characterization and antimicrobial susceptibility patterns of 54 V. harveyi strains collected from Larimichthys crocea in China. The isolates displayed resistance to a variety of antibiotics and carried multiple virulence genes. Additionally, a novel V. harveyi-specific phage with broad host range was discovered and showed high efficacy in preventing and controlling V. harveyi infection in a zebrafish model.

AQUACULTURE (2021)

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Bacteriophage isolated from non-target bacteria demonstrates broad host range infectivity against multidrug-resistant bacteria

Lyman Ngiam et al.

Summary: Antibiotic resistance is a global health challenge, and phage therapy, as an alternative treatment to antibiotics, can be more effective by targeting commensal Escherichia coli as hosts to isolate phage with greater therapeutic potential.

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Application of a novel phage vB_SalS-LPSTLL for the biological control of Salmonella in foods

Yating Guo et al.

Summary: Phage LPSTLL is able to lyse 11 Salmonella serotypes, has broad lytic range, and can effectively suppress Salmonella growth in liquid culture. It shows rapid reproductive activity with wide applicability for food processing and storage. LPSTLL is a novel member of a new genus, with potential for Salmonella biocontrol in the food industry.

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Coevolutionary phage training leads to greater bacterial suppression and delays the evolution of phage resistance

Joshua M. Borin et al.

Summary: Training bacteriophages on target hosts before therapy can prolong suppression of antibiotic-resistant bacteria by delaying the evolution of resistance. Evolution of resistance to trained phages is more costly and requires multiple mutations, making them more effective in combating resistance. Transfer of genetic information from host genomes through recombination can enhance phage fitness and improve treatment outcomes, highlighting the potential of training in phage therapy development.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

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Screening of a Plesiomonas shigelloides phage and study of the activity of its lysis system

Jingdan Zhang et al.

Summary: Plesiomonas shigelloides is a significant fish pathogen in aquaculture and the PSP01 phage has shown promising lytic activity against it, providing a new tool for controlling fish pathogens. The phage exhibits good activity over a wide range of conditions, indicating its potential for aquaculture applications.

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Bacteriophage Therapy for Critical and High-Priority Antibiotic-Resistant Bacteria and Phage Cocktail-Antibiotic Formulation Perspective

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Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout

Heidi M. T. Kunttu et al.

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Olatz Larranaga et al.

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Quanxin Gao et al.

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Sublethal streptomycin concentrations and lytic bacteriophage together promote resistance evolution

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Synergistic antimicrobial activity of bacteriophages and antibiotics against Staphylococcus aureus

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Phage selection restores antibiotic sensitivity in MDR Pseudomonas aeruginosa

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Virome-associated antibiotic-resistance genes in an experimental aquaculture facility

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Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp.

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Temperature-induced activation of freshwater Cyanophage AS-1 prophage

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Bacteriophage endolysins: A novel anti-infective to control Gram-positive pathogens

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