期刊
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
卷 60, 期 2, 页码 167-178出版社
VIA MEDICA
DOI: 10.5603/FHC.a2022.0015
关键词
Adipose mesenchymal stem cell-derived exosomes; 0-chitin nanofiber; rat skin; wound healing; transcriptomics; proteomics
资金
- Department of Science and Technology of Liaoning Province [2019010205, 2021JH2/10300024]
- PLA Foundation of China [CLB20C036]
The study found that AMSC-exos-loaded 0-ChNF hydrogel can promote wound healing by acting on CFD and downstream proteins, accelerating wound closure and re-epithelialization process.
Introduction. Clarifying the role and mechanism of exosome gel in wound repair can provide a new effective strategy for wound treatment. Materials and methods. The cellular responses of adipose mesenchymal stem cell-derived exosomes (AMSC--exos)and the wound healing ability of AMSC-exos-loaded 0-chitin nanofiber (0-ChNF) hydrogel were studied in vitro in mouse fibroblasts cells (L929) and in vivo in rat skin injury model. The transcriptome and proteome of rat skin were studied with the use of sequenator and LC-MS/MS, respectively. Results. 80 and 160 mu g/mL AMSC-exos could promote the proliferation and migration of mouse fibroblastic cells. Furthermore, AMSC-exos-loaded 0-ChNF hydrogel resulted in a significant acceleration rate of wound closure, notably, acceleration of re-epithelialization, and increased collagen expression based on the rat full-thickness skin injury model. The transcriptomics and proteomics studies revealed the changes of the expression of 18 genes, 516 transcripts and 250 proteins. The metabolic pathways, tight junction, NF -KB signaling pathways were en-riched in Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway. Complement factor D (CFD) and downstream Aldolase A (Aldoa) and Actn2 proteins in rats treated with AMSC-exos-loaded 0-ChNF hydrogel were noticed and further confirmed by ELISA and Western blot. Conclusion. These findings suggested that AMSC-exos-loaded 0-ChNF hydrogel could promote wound heal-ing with the mechanism which is related to the effect of AMSC-exos on CFD and downstream proteins. (Folia Histochemica et Cytobiologica 2022, Vol. 60, No. 2, 167-178)
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