期刊
FISH & SHELLFISH IMMUNOLOGY
卷 125, 期 -, 页码 285-291出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.05.027
关键词
Long non -coding RNA; LncRNA; MITA; Gene regulation; Antiviral; Innate immune
资金
- National Key Research and Devel- opment Project [2018YFD0900301]
- National Nature Science Foundation of China [31822057]
This study reports a novel lncRNA, LTCONS7822, in miiuy croaker that plays a positive regulatory role in antiviral immunity. The lncRNA was found to positively regulate MITA at the posttranscriptional level. The study provides new information on the immune regulation network of lncRNA in teleost fish.
Accumulated studies have shown that long non-coding RNA (lncRNA) is considered a critical regulatory factor in mammals, with a length greater than 200 nucleotides, and it can participate in gene imprinting, dose compensation, transcription enhancement, and antisense regulation. Most of the above studies are carried out in mammals, and there are very few studies on lncRNA of lower vertebrates. Here, we report a novel lncRNA, LTCONS7822, which can play a positive regulatory effect on antiviral immunity in miiuy croaker, Miichthys miiuy. Our results show that the levels of lncRNA LTCONS7822 were significantly increased after poly (I:C) stimulation. Further study, we found that lncRNA LTCONS7822 could positively regulate MITA at the posttranscriptional level. In addition, the dual-luciferase reporter assay analysis showed that the positive regulatory effect of lncRNA LTCONS7822 on NF-kappa B and IRF3 signaling pathways presented the dose and time-dependent manner. Western blotting experiments proved that lncRNA LTCONS7822 has a positive regulatory effect on MITA. Collectively, our study provided new information to enrich the immune regulation network of lncRNA in teleost fish.
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