期刊
FISH & SHELLFISH IMMUNOLOGY
卷 124, 期 -, 页码 462-471出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.04.039
关键词
Epinephelus coioides; Sec6; SGIV; Replication; Apoptosis
资金
- Key-Area Research and Development Program of Guangdong Province [2021B0202040002]
- Laboratory of Lingnan Modern Agriculture Project [NT2021008]
- Natural Science Foundation of Guangdong, China [2022A1515010648]
- National Natural Science Foundation of China [U20A20102, 31930115, 31772877, 31330082, 41876162, 41706186]
- Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) [311021006]
- Marine Fisheries Bureau Key Funds and Marine Projects [GDME-2018C002]
- National Key R&D Program of China [2018YFC0311300, 2018YFD0900501, 2018YFC0311302]
- China Agricultural Research System [CARS-47-G16]
- China Modern Agricultural Industry Technology System (The Control of Parasites Infection on Marine Fish) [CARS-47-18]
- National Key Research and Development Plan Subject [2019YFD0900801, 2020YFD0901100]
Sec6, a component of the exocyst complex, plays important roles in cellular functions and SGIV infection, and regulates apoptosis.
Exocyst complex component 3 Sec6 of mammals, one of the components of the exocyst complex, participates in numerous cellular functions, such as promoting cell migration and inhibiting apoptosis. In this study, the Sec6 was obtained from Epinephelus coioides, an economically important cultured fish. The full length of E. coioides Sec6 was 2655 bp including a 245 bp 5 ' UTR, a 154 bp 3 ' UTR, and a 2256 bp open reading frame (ORF) encoding 751 amino acids, with a molecular mass of 86.76 kDa and a theoretical pI of 5.57. Sec6 mRNA was detected in all the tissues examined, but the expression level is different in these tissues. Using fluorescence microscopy, Sec6 were distributed in both the nucleus and the cytoplasm. After SGIV infection, the expression of E. coioides Sec6 was significantly up-regulated in both trunk kidney and spleen response to Singapore grouper iridovirus (SGIV), an important pathogens of E. coioides. Sec6 could increase the SGIV-induced cytopathic effects (CPE), the expression of the SGIV genes VP19, LITAF, MCP, ICP18 and MCP, and the viral titers. Besides, E. coioides Sec6 significantly downregulated the promoter of NF-kappa B and AP-1, and inhibited the SGIV-induced apoptosis. The results demonstrated that E. coioides Sec6 might play important roles in SGIV infection.
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