期刊
FISH & SHELLFISH IMMUNOLOGY
卷 122, 期 -, 页码 215-224出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.01.020
关键词
Nile tilapia; Rambutan seed; Innate immunity; Growth performance; Gene expression
资金
- National Research Council of Thailand
- Chiang Mai University
This study demonstrates that rambutan seed can be used as a feed additive in Nile tilapia raised under a biofloc system, resulting in significant improvements in growth, skin mucus, serum immune parameters, and immune-antioxidants related gene expressions.
An eight-week feeding trial was carried out to determine the effects of rambutan seed (RS) as a feed additive on the growth, skin mucus, serum immune parameters, and gene expression of Nile tilapia (Oreochromis niloticus) raised under a biofloc system. Nile tilapia fingerlings (14.77 & PLUSMN; 0.80 g fish(-1)) were fed five experimental diets containing 0, 5, 10, 20, and 40 g kg(-1) of RS, corresponding to five treatments (RS0, RS5, RS10, RS20, and RS40) with three replications per treatment. The results showed that fish consuming the RS10 and RS20 diets presented a substantial (P < 0.05) improvement in specific growth rate (SGR), weight gain (WG), and feed conversion ratio (FCR) after eight weeks. The highest values were recorded in the RS10 diet; however, there were no significant (P > 0.05) differences exhibited in the fish survival rates between treatments. The RS supplementation diets demonstrated greater immunological parameters, particularly skin mucus and serum immune responses (P < 0.05), than that of the control after eight the eight-week feeding trial. The highest level was seen in fish fed the RS10; followed by the RS20, RS40 (P > 0.05), and RS5 diets. Regarding gene expressions, IL1, IL8, LBP, GSTa, and GSR genes were significantly up-regulated in fish provided the RS10 diet in comparison to the control and other supplemented diets (P < 0.05). However, no significant up-regulation was found in these genes among the RS0, RS5, RS20, and RS40 diets, with the exception of the GPX gene. Similarly, up-regulation of IL-8, LBP, GSTa, GPX, and GSR were noted in fish fed the RS10 diet (P < 0.05). Notably, no significant differences were evident in these genes among the RS5, RS20, and RS40 diets. In conclusion, fish fed RS10 (10 g kg(-1)) significantly enhanced growth, skin mucus, serum immunities, and immune-antioxidants related gene expressions of Nile tilapia raised under biofloc system.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据