microRNA-122 regulates NF-κB signaling pathway by targeting IκBα in miiuy croaker, Miichthys miiuy

The inhibitory protein I kappa B alpha plays a key role in the inflammatory process and immune response by regulating the activity of the transcription factor NF-kappa B. microRNA (miR) is a small non-coding RNA that can regulate many biochemical processes, such as cell growth, proliferation, and immune response. In this study, it was first predicted that I kappa B alpha is the target of miR-122 through bioinformatics, and it was confirmed by dual fluorescence experiments. Then we found that miR-122 can inhibit the expression of I kappa B alpha at the mRNA and protein levels, thereby promoting the p65-activated NF-kappa B pathway. It is speculated that miR-122 plays an important role in the innate immunity of teleost fish. This study will help to further understand miRNAs regulatory mechanism in teleost fish.

Automated summary

This study finds that miR-122 can promote the activation of NF-kappa B pathway by regulating the expression of I kappa B alpha, thus playing an important role in the immune response of teleost fish.