期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 922, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2022.174890
关键词
Genetically modified rabbit; Gene targeting; Animal model; Cardiovascular medicine
资金
- Scientific Research Plan Project of Education Department of Shaanxi Province [21JK0883]
- Science and Technology Innovation Base-Open and Sharing Platform of Science and Technology Resources Project of Shaanxi Province [2019 PT-26]
- Supporting Funds for the Construction of Shared experimental Platform for Basic Medicine and Translational Medicine of Xian Medical University [2021PT06]
- Doctoral Initiation Fund Project [2021DOC14]
Genetically modified (GM) rabbits expressing human genes have been widely used as animal models for studying cardiovascular diseases. Current methods for gene modification in rabbits include prokaryotic microinjection and RNA interference, but stable ES cell lines are lacking. However, with the emergence of new genetic technologies such as ZFNs, TALENs, CRISPR, and CRISPR/Cas9, significant progress has been made in rabbit gene targeting.
Genetically modified (GM) rabbits are outstanding animal models for studying human genetic and acquired diseases. As such, GM rabbits that express human genes have been extensively used as models of cardiovascular disease. Rabbits are genetically modified via prokaryotic microinjection. Through this process, genes are randomly integrated into the rabbit genome. Moreover, gene targeting in embryonic stem (ES) cells is a powerful tool for understanding gene function. However, rabbits lack stable ES cell lines. Therefore, ES-dependent gene targeting is not possible in rabbits. Nevertheless, the RNA interference technique is rapidly becoming a useful experimental tool that enables researchers to knock down specific gene expression, which leads to the genetic modification of rabbits. Recently, with the emergence of new genetic technology, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR), and CRISPR-associated protein 9 (CRISPR/Cas9), major breakthroughs have been made in rabbit gene targeting. Using these novel genetic techniques, researchers have successfully modified knockout (KO) rabbit models. In this paper, we aimed to review the recent advances in GM technology in rabbits and highlight their application as models for cardiovascular medicine.
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