Probiotic supplementation alleviates absence seizures and anxiety- and depression-like behavior in WAG/Rij rat by increasing neurotrophic factors and decreasing proinflammatory cytokines

Aim: Epilepsy is one of the most common chronic brain disorders that affect millions of people worldwide. In the present study, we investigated the effects of probiotic supplementation on absence epilepsy and anxiety-and depression-like behavior in WAG/Rij rats. Material and method: Fourteen male WAG/Rij rats (absence-epileptic) and seven male Wistar rats (non epileptic) were used. The effects of probiotic VSL#3 (12.86 bn living bacteria/kg/day for 30 day/gavage) on absence seizures, and related psychiatric comorbidities were evaluated in WAG/Rij rats. Anxietylike behavior was evaluated by the open-field test and depression-like behavior by the forced swimming test. In addition, the brain tissues of rats were evaluated histopathologically for nerve growth factor [NGF], brain-derived neurotrophic factor [BDNF], SRY sex-determining region Y-box 2 [SOX2] and biochemically for nitric oxide [NO], tumor necrosis factor-alpha [TNF-a] ,and Interleukin-6 [IL-6]. Results: Compared to Wistar rats, WAG/Rij rats exhibited anxiety- and depression-like behavior, and had lower BDNF, NGF and SOX2 immunoreactivity, and higher TNF-a, IL-6 levels in brain tissue. VSL#3 supplementation reduced the duration and number of spike-wave discharges (SWDs) and exhibited anxiolytic or anti-depressive effect. VSL#3 supplement also increased the NGF immunoreactivity while decreasing IL-6, TNF-a and NO levels in WAG/Rij rat brain. Conclusion: The findings of the present study showed that neurotrophins, SOX2 deficiency, and proinflammatory cytokines may play a role in the pathogenesis of absence epilepsy. Our data support the hypothesis that the probiotics have anti-inflammatory effect. The present study is the first to show the positive effects of probiotic bacteria on absence seizures and anxiety- and depression-like behavior. (c) 2022 Elsevier Inc. All rights reserved.

Automated summary

This study investigated the effects of probiotic supplementation on absence epilepsy and anxiety- and depression-like behavior. The results showed that VSL#3 supplement reduced the duration and number of seizures and had anxiolytic and anti-depressive effects. Additionally, VSL#3 supplementation increased the immunoreactivity of neurotrophic factors and decreased the levels of proinflammatory cytokines.